Transformation point is a tiny circle displayed on the symbol. This point is used when transforming the symbol. In other words, the point where you rotate the symbol. You can move this point around using the Transformation tool.
Registration point is a tiny cross-hair used to set the coordinate of the symbol on the stage. When a symbol is created, it defaults to the top left corner of the symbol. This is the same as a symbol’s current X/Y coordinate. X = Distance from the left of the stage. Y = Distance from the top of the stage. This point can be manipulated via ActionScript code. For example, the point, or X/Y coordinate is used to determine collision.